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      Dionex DNAPac PA100 寡核苷酸色譜柱

      更新時間:2019-11-12

      簡要描述:

      Dionex DNAPac PA100 寡核苷酸色譜柱
      特點是采用強陰離子交換樹脂,從而可對合成型和經過修飾的寡核苷酸進行高分辨率分析和純化。

      Dionex  DNAPac PA100 寡核苷酸色譜柱

      Thermo Scientific? Dionex? DNAPac? PA100 寡核苷酸柱的特點是采用強陰離子交換樹脂,從而可對合成型和經過修飾的寡核苷酸進行高分辨率分析和純化。

       

      描述

      • 13μm、薄膜型、無孔、聚合物樹脂,并帶有與季胺官能團鍵合的 MicroBeads?

      • 可對寡核苷酸進行 n、n-1 分離

      • 解析次級結構的寡核苷酸

      • 與溶劑、高 pH 和高溫相容

      • 分析基于硫代磷酸酯的臨床樣品

      • 可輕松升級

       

      Dionex  DNAPac PA100 寡核苷酸色譜柱

      訂貨信息:

      分析柱
      DNAPac PA-100 Analytical Column (4 x 250 mm)043010
      DNAPac PA-100 Semi-Preparative Column (9 x 250 mm)043011
      DNAPac PA-100 Semi-Preparative Column (22 x 250 mm)SP2091
      FAST-Cartridge, DNAPac PA-100 (2 x 10 mm), Pkg of 150SP4008
      FAST-Cartridge, DNAPac PA-100 (2 x 10 mm), Pkg of 600SP3229
      DNA Pac PA-100 Analytical Column (2 x 250 mm)SP3686
      DNAPac PA-100 Column (22 x 50 mm)SP4513
      FAST-Cartridge, DNAPac PA-100 (2 x 10 mm), Pkg of 10SP4757
      保護柱
      DNAPac PA-100 Guard Column (2 x 50 mm)SP4016
      DNAPac PA-100 Guard Column (4 x 50 mm)043018

       

       

      For n, n-1 Resolution of Single-Stranded Oligonucleotides

      The DNAPac? PA100 is a high-resolution anion-exchange column that provides single-base resolution. It is stable under denaturing conditions, rugged, reliable, and can be readily scaled up. The DNAPac PA100 is a 13 μm pellicular, nonporous polymeric resin with bound quaternary amine-functionalized MicroBeads?. The rapid mass-transport characteristics of this resin result in very high-resolution oligonucleotide separations.

      • Capable of n, n-1 resolution for oligonucleotides

      • Resolves oligonucleotides with secondary structures

      • Compatible with solvent, high pH, and high temperatures

      • Analyzes phosphorothioate-based clinical samples

      • Provides easy scale-up

      The MicroBead resin used in DNAPac columns provides high-resolution separations to ensure maximum peak purity, and makes linear scale-up of your separation easy. For more complex separations, the polymeric properties of the DNAPac PA100 allows great flexibility to select ideal conditions, including high pH, high temperature, or chaotropic agents.

      Synthetic oligonucleotides can be screened for production yield and failure sequences using the DNAPac PA100. Unit-base resolution of synthetic oligonucleotides to 60 bases and beyond has been demonstrated. The DNAPac PA100 can even be operated under denaturing conditions. Either high temperature (up to 90 °C) or high-pH eluents (up to pH 12.5) can be used to eliminate hydrogen bonding, allowing resolution of problem sequences such as self-complementary sequences or poly-G stretches.

      Separates RNA / Double-Stranded DNA

      The DNAPac PA100 is ideally suited for the purification and analysis of synthetic RNA. Failure sequences are easily separated from the full-length product. Double-stranded DNA, such as plasmids, or restriction fragments are also separated.

      Analytical separations on the 4 mm diameter column can be scaled directly to larger diameter columns so preparative methods can be conveniently developed using small samples. The loading capacity of the DNAPac PA100 (4 × 250 mm) column is from 1 to 100 μg. Scaling the flow rate and sample size up for the 9 × 250 mm and 22 × 250 mm column yields essentially identical chromatography.

      DNAPac? PA100 for n, n - 1 Resolution of Single-Stranded Oligonucleotides Specifications
      Temperature range4–90 °C
      Typical operating conditions2400 psi at 1.0 mL/min (analytical and guard columns)
      Recommended flow rate: 1.0 mL/min
      Mobile phase compatibilitypH 2–12.5; 100% compatible with common organic solvents.
      Ionic form eluents: chloride, acetate, or bromide in lithium, sodium, or ammonium forms.
      Resin composition13 μm-diameter nonporous substrate (55% cross-linking) agglomerated with a 100 nm MicroBead alkyl quaternary ammonium functionalized latex (5% cross-linked).
      Anion-exchange capacity~40 μeq/column (4 × 250 mm analytical column)
      Column constructionPEEK? with 10-32 threaded ferrule-style end fittings.
      All components are nonmetallic.
      DimensionsDNAPac PA100 Analytical Column:
      2 × 250 mm

      DNAPac PA100 Guard Column:
      2 × 50 mm

      DNAPac PA100 Analytical Column:
      4 × 250 mm

      DNAPac PA100 Guard Column:
      4 × 50 mm

      DNAPac PA100 Semipreparative Column:
      9 × 250 mm

      DNAPac PA100 Semipreparative Column:
      22 × 250 mm

      DNAPac PA100 Semipreparative Guard Column:
      22 × 50 mm
      Maximum operating pressure4000 psi (27.9 MPa)

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