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      產品中心您現在的位置:首頁 > 產品展示 > 通用色譜配件 > 行業專用色譜柱 > Dionex AminoPac PA10氨基酸分析色譜柱

      Dionex AminoPac PA10氨基酸分析色譜柱

      更新時間:2019-11-12

      簡要描述:

      Dionex AminoPac PA10氨基酸分析色譜柱
      AminoPac PA10柱結合陰離子交換,梯度淋洗以及脈沖電化學檢測,可以分離多種氨基酸。

      Dionex AminoPac PA10氨基酸分析色譜柱

      用途和特點
      AminoPac PA10柱結合陰離子交換,梯度淋洗以及脈沖電化學檢測,可以分離多種氨基酸。分離氨基酸的同時,還可檢測氨基糖及碳水化合物。此外,還可分離多種糖類,磷酸化氨基酸,以及含硫氨基酸的普通氧化產物,如磺基丙氨酸。其柱填料為疏水性高聚物薄殼型陰離子交換劑,可在pH0-14范圍內使用。填料*的pH穩定性允許使用的淋洗液,有助于氨基酸在金電極的陽極氧化。

       

       

      Dionex AminoPac PA10氨基酸分析色譜柱

      訂貨信息:

      分析柱
      AminoPac PA10 Analytical Column (2 x 250 mm)055406
      AminoPac PA10 (22 x 250 mm)SP5488
      AminoPac PA10 (9 x 250 mm)SP5490
      AminoPac PA10 Analytical Column (4 x 250 mm)SP5678
      保護柱
      AminoPac PA10 Guard Column (2 x 50 mm)055407
      AminoPac PA10 Guard Column (4 x 50 mm)SP5680
      配件
      AAA-Direct Installation Kit059539
      Carbohydrate Removal Cartridge (CRC) for AAA-Direct068598

       

      For High-Resolution Separations of Free Amino Acids by AAA-Direct

      The AminoPac® PA10 column is packed with a hydrophobic, polymeric, pellicular, anion-exchange resin that is stable over the range of pH 0–14. The unique pH stability allows the use of eluents that are conducive to anodic oxidation of amino acids at gold electrodes. This column is recommended for use with the AAA-Direct Amino Acid Analyzer, allowing direct detection of primary and secondary amino acids by IPAD, with no need for pre- or postcolumn derivatization.

      • Derivatization not required

      • Carbohydrates and amino acids separated and detected simultaneously

      • Mid-femtomole to low-picomole detection limits

      • Compatible with all commonly used hydrolysis procedures

      • Can be configured for on-line analysis

      AAA-Direct is a technique that eliminates the need for any form of derivatization, and is therefore compatible with on-line monitoring of cell cultures and fermentation broths. 

      The AminoPac PA10 column is optimized for separations of free amino acids by AAA-Direct. Vitamins, amino sugars, and carbohydrates can be detected simultaneously with amino acids. Additional capabilities include separation of a wide range of sugars, phosphorylated amino acids, and common oxidation products of sulfur-containing amino acids, such as cysteic acid, methionine sulfone, and methionine sulfoxide.

       

       

      AminoPac PA10 Column for Amino Acid Analysis by AAA-Direct  Specifications
      Recommended operating temperature30 °C
      Typical operating conditions0.25 mL/min gradient
      Recommended flow rate: 0.25 mL/min
      Mobile phase compatibilitypH 0–14; 100% compatible with common organic solvents.
      Dionex sodium acetate ionic form eluents only.
      Resin composition8.5-μm-diameter microporous (<10 ?) bead (55% cross-linked)
      agglomerated with an 80-nm microbead alkyl ammonium functionalized latex (1% cross-linked).
      Anion-exchange capacity60 μeq/column (2 × 250 mm analytical column)
      Column constructionPEEK with 10-32 threaded ferrule-style end fittings.
      All components are nonmetallic.
      DimensionsAminoPac PA10 Analytical Column:
      2 × 250 mm
      AminoPac PA10 Guard Column:
      2 × 50 mm
      Maximum operating pressure4000 psi (27.9 MPa)

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