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      產品中心您現在的位置:首頁 > 產品展示 > 通用色譜配件 > 行業專用色譜柱 > Dionex AminoPac PA1 氨基酸分析色譜柱

      Dionex AminoPac PA1 氨基酸分析色譜柱

      更新時間:2019-11-12

      簡要描述:

      Dionex AminoPac PA1 氨基酸分析色譜柱
      特別適用于分析酸性和酸不穩定性氨基酸,以及用陽離子交換分離不*的氨基酸對。

      Dionex AminoPac PA1 氨基酸分析色譜柱

      用途和特點
      AminoPac PA1柱用于氨基酸的快速分離。特別適用于分析酸性和酸不穩定性氨基酸,以及用陽離子交換分離不*的氨基酸對。

       

       

      Dionex AminoPac PA1 氨基酸分析色譜柱

      訂貨信息:

      分析柱
      AminoPac PA1 Analytical Column (4 x 250 mm)037022
      保護柱
      AminoPac PA1 Guard Column (4 x 50 mm)037023

       

       

      For Specialized Amino Acid Applications with Postcolumn Derivatization

      The AminoPac® PA1 column is a high-speed, pellicular, strong, anion-exchange column. The PA1 delivers high-efficiency separations as a result of its nonporous-core particle. Its strong anion-exchange characteristics are imparted by agglomerating surface MicroBead™ particles that are functionalized with the quaternary ammonium functional group, resulting in a higher capacity than typically offered by nonporous resins.

      • Resolves phosphorylated and other amino acids by anion-exchange chromatography

      • Separates acid-labile amino acids under basic pH conditions

      • Separates strongly acidic amino acids

      • Requires OPA or ninhydrin postcolumn derivatization

      The anion-exchange selectivity of the AminoPac PA1 can be used to resolve peaks that cannot be resolved using a cation-exchange column. For example, O-phosphorylated amino acids are resolved and eluted near the end of a hydrolysate chromatogram. Arogenic acid, a precursor to tyrosine and phenylalanine, decomposes under the acidic conditions required for cation-exchange separation or PITC derivatization, but it is stable in alkaline eluents used with AminoPac PA1 column.

      The PA1 is particularly well suited for acidic and acid-labile amino acids and for amino acid pairs not completely resolved by cation-exchange chromatography.

       

       

       

      AminoPac PA1 Column for Specialized Amino Acid Applications with Postcolumn Derivatization  Specifications
      DimensionsAminoPac PA1 Analytical Column:
      4 × 250 mm
      AminoPac PA1 Guard Column:
      4 × 50 mm
      Maximum operating pressure4000 psi (27.9 MPa)
      Recommended operating temperatureAmbient
      Typical operating conditions1.0 mL/min gradient
      Recommended flow rate: 1.0 mL/min
      Mobile phase compatibilityCompatible with up to 5% common organic solvents.
      Recommended eluents: hydroxide, borate, and acetate.
      Resin composition10-μm-diameter, pellicular substrate (2% cross-linked)
      agglomerated with a 180 nm MicroBead alkyl quaternary ammonium functionalized latex (20% cross-linked).
      Anion-exchange capacityN/A
      Column constructionPEEK with 10-32 threaded ferrule-style end fittings.
      All components are nonmetallic.

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